Detection of Escherichia coli O157:H7
What is E. coli O157:H7? (Click here)
DNA BASED METHODS

Warnex E. coli O157 Assay (Warnex Diagnostics) CCD

Warnex E. coli O157:H7 Assay (Warnex Diagnostics) CCD
Real-Time PCR Technology
Specific results in under 3 h post-enrichment
NR

GENE-TRAK (Gene-trak)

Nucleic acid Hybridization
24-48h
NR

BAX system E.coli O157(Qualicon) CCD
PCR
After enrichment, 4 h for only test
NR

TAQMAN (PE Applied Biosystems)
PCR
NR
NR

IMMUNOASSAY BASED METHODS

Pathatrix (Matrix Microscience Inc.) CCD
antibody coated paramagnetic particles
NR
NR

E. coli O157 Test Kits (Hardy Diagnostics)
CCD
EIA
NR
NR


Assurance EIA EHEC (BioControl)
CCD
ELISA
<20 h                              
NR

Premier EHEC (Meridian Diagnostics)
ELISA
3 min for only test
NR

EZ Coli (Becton Dickison; cf Difco)
ELISA              
24 h                   
$9.48


ANTI E.coli O157 (Dynal Inc.)

immunomagnetic separation
30 h
$2.60

REVEAL E.coli O157:H7(Neogen Co.)
CCD
Immunoassay
6-28h
$9.50

Transia Card E.coli O157 (GENE-TRAK.)
Immunochromatographic card
NR
NR

EiaFoss E.coli O157 (Foss Electric A/S)
Immunoassay
NR
NR

E. coli O157 detection kits
(Microgen Bioproducts, Co.)
CCD
NR
NR

Rapid Check (Strategic Diagnostics Inc.) CCD
Immunoassay based method
NR

VIDAS (bioMerieux Vitek) CCD
ELFA
24-48 h
NR

mini-VIDAS (bioMerieux Vitek) CCD
ELFA
24-48 h
NR

EHEC-Tek (Organon Tecknika)
ELISA
20-26 h
NR

EHEC O157-ELISA (3M)
Blot ELISA
26-28 h
$5.00

VIP EHEC (BioControl)   CCD
immunoprecipitation             
18 h      
$8.50

E.coli O157:H7 Via kit (TECRA) CCD
ELISA
20 h                           
$6.00

E. coli O157 Immunocapture (TECRA)
CCD
ELISA
NR
NR

Path-Stik E.coli O157 (Celsis) CCD
Immunochromatographic
10 min
NR

IMMUNO-LATEX AGGLUTINATION BASED METHODS
E.coli O157 Latex test (Oxoid)
Latex agglutination
3-5 min for only test    
NR

DRYSPOT E.coli O157 Latex test (Oxoid)
Latex agglutination
3-5 min for only test    
NR


VET-RPLA (Unipath Ltd.)

Latex agglutination
NR         
$11.04

E.coli Confirmation (HardyDiagnostics)

Latex agglutination
NR
$0.86

E.coli O157 Latex Test (Unipath Ltd.)
Latex agglutination
2 min for only test           
NR

E.coli O157 Latex test (Remel) CCD
Latex agglutination
3-5 min for only test
NR

ImmunoCard STAT E.coli O157
(Meridian Diagnostics)

Latex agglutination
NR
NR
BIOCHEMICAL METHODS AND OTHERS
Rapidec.coli (bioMerieux Vitek) CCD
beta-D-glucuronidase
2-6 h for only test      
NR
Rainbow Agar (Biolog)
NR
24 h
NR


Bactident E. coli (Merk)

beta-D-glucuronidase
30 min for only test

NR
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