Bio-Rad Receives AOAC Approval for Detection of Salmonella in Pet Food and Environmental Surfaces
Bio-Rad Laboratories iQ-CheckTM Salmonella II kit has received AOAC approval for real-time PCR detection of Salmonella from wet cat food, dry dog food, stainless steel, plastic, ceramic and concrete. This represents a Performance Tested MethodSM matrix extension for the iQ-Check Salmonella II kit, which is currently approved for raw chicken, raw beef, eggs, cantaloupe and peanut butter.
Over 23,000 tons of pet food was recalled between 2006 and 2008, according to a study conducted by the Centers for Disease Control and Prevention (CDC). Of the 79 human patients infected with Salmonella as a result of contaminated pet food, 48% of them were children under the age of 2.
The performance of the iQ-Check kit was compared to the FDA Bacteriological Analytical Manual (BAM) reference method for detection of Salmonella from the pet food and environmental surfaces. There was no significant difference between the performance of two methods. All samples that were detected as positive by iQ-Check were confirmed by the reference method.
The method involves a single primary 20hr enrichment in buffered peptone water followed by a one step high throughput DNA extraction in 96-well plate format. With a PCR run time of about 2hr, complete results are available in less than 24hr. Highly sensitive and specific DNA probes developed by scientists at Bio-Rad allow for accurate and rapid real-time PCR detection of Salmonella with no interference from common cross reacting organisms.
Deborah McKenzie, Senior Director of the AOAC Research Institute commented that “AOAC method validation brings the value of an independent third party to the evaluation process. The AOAC Research Institute, a subsidiary of AOAC INTERNATIONAL, administers the Performance Tested Methods program. Method developers like Bio-Rad, who submit methods in the AOAC Performance Tested Methods program, recognize the added value to their products that an independent third party evaluation and the certification mark provide.”
“Our extensive experience with quantitative PCR technology coupled with a deep appreciation of the challenges facing food pathogen detection come together in our iQ Check product line,” says Brad Crutchfield, Bio-Rad Group Vice President. He went on to say, “The result is a fast and reliable tool that allows our customers to make better decisions. It doesn’t get any simpler than that.”
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